FastQCFastQC Report
Sun 24 Apr 2016
G130-M11_CCGTCC_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M11_CCGTCC_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10405509
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA9050.012.39722827
GGCGAAT5000.011.8889441
CTCGAAT16300.011.8862421
TCGGTTT17200.011.5111612
CCCGTAT5750.011.4869021
AATTGCG9700.011.11407336
GTCGGTT17250.010.9763731
CGGGGAT11600.010.4388881
CGCGTAG2751.3215958E-610.4078291
CGGGCTA6300.010.1346081
CGCCCTA2403.5307894E-510.0909251
CGAATGT15550.010.0446083
ATTGCGA10750.09.82384837
CGCGTAT2302.5556344E-49.5724181
GTCCGTA4907.6397555E-119.435671
TGCGATA11250.09.38732339
CTCGATT7800.09.31471
CGGTTTG20450.09.2514573
TAAGGCG16850.09.0084929
AACGTGC10450.08.84170319
Produced by FastQC (version 0.11.2)