FastQCFastQC Report
Sun 24 Apr 2016
G130-M11_CCGTCC_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M11_CCGTCC_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10405509
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT17750.011.1567511
CACGATA12100.010.18113142
CCACTAG22100.010.1537473
CGATACA12300.09.83682944
CGATCAC5253.6379788E-129.63800053
CGCAATT11000.09.6015661
ACGATAC13400.09.52174643
TAGCTCG13300.09.42943122
CGGAATT17300.09.2844852
TCACGAT14550.09.07155241
CGTAAAC3004.190028E-58.8014351
CACGATC8300.08.7484161
CGACAAT6301.8189894E-128.7315841
CGGGTAT8600.08.4432381
CGTGGAT14400.08.4041491
TACCGGT10850.08.31280210
CGAATAA7150.08.30836920
GGAAGAT67700.08.2554091
TTCGATC7200.08.2511082
GGGGGAT14850.08.1494781
Produced by FastQC (version 0.11.2)