FastQCFastQC Report
Sun 24 Apr 2016
G130-M12_GTCCGC_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M12_GTCCGC_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14707295
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT5400.011.0085121
CTCGAAT21550.010.4210491
GTCGGAT9200.09.5726181
CCGGAAT12100.09.0979411
CGCGGTT5701.8189894E-118.8840611
ACGATCG4002.1557753E-78.7996833
CTACGCG2250.00223984948.7996239
TAAGGCG19750.08.6882359
CTCGTTT25900.08.6707951
CCCGTAT11100.08.5291161
AATTGCG10200.08.41217736
GGCGTAT13350.08.0811541
ACCGGTA10950.08.03618427
CCCGGAT20600.08.0159061
GTCGAAT12400.07.99004751
CGAATGT20150.07.96993543
TCGGTTT23200.07.96522952
CGGGCTA7205.456968E-127.95059061
CGGGCAT22200.07.9340611
CCGGGAT16100.07.93159771
Produced by FastQC (version 0.11.2)