FastQCFastQC Report
Sun 24 Apr 2016
G130-M12_GTCCGC_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M12_GTCCGC_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14707295
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT22900.011.6257071
CGATACA15850.011.10363744
CGACGAT8600.010.2336611
CGGGGAT23250.010.2204551
CGGAATT22200.010.1088192
ACGATAC17200.09.97615743
CACGATA17000.09.9641242
TACGCAA9750.09.4765599
ACGCAAC10250.09.22903810
GGGAAAT49250.08.9796471
GACGATC12900.08.6982862
TCACGAT20100.08.64625741
AATCGTG12650.08.3478165
CGTATTA9650.08.20704910
CCACTAG30400.08.1052823
CCCCGTA10900.08.0730847
CGGAAAT21000.08.0675361
CCGTTAA9950.07.960034418
TTATCCG9150.07.9341756
GGGGGAT22200.07.92878251
Produced by FastQC (version 0.11.2)