FastQCFastQC Report
Sun 24 Apr 2016
G130-M14_GTGGCC_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M14_GTGGCC_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9564798
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT17850.013.5684191
CGAATGT15550.011.6008033
CCCGTAT5400.011.4166611
ATCCGAA11900.011.27684225
CTCGCAT6450.010.9235831
TCGCATT4551.8189894E-1210.636922
AATTGCG8300.010.60285636
ATTGCGA8950.010.57027837
CTCCGTT21000.010.1701491
GTCGGTT15050.010.0945611
ACCGGTA8950.010.07779427
CGGGCTA4602.1827873E-1110.0516251
CTCGTAT5950.09.9912921
TCCGATT16150.09.9438582
TTCGATT13300.09.9243992
GTTCGAT13800.09.89207651
AATCCGA13350.09.8872324
TCGAATG17550.09.652022
TCGGTTT19000.09.61012652
TTGCGAT9650.09.575582538
Produced by FastQC (version 0.11.2)