FastQCFastQC Report
Sun 24 Apr 2016
G130-M16_CGTACG_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M16_CGTACG_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12528480
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152790.12195413968813455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT19400.013.165031
CGGGCTA5900.012.6879791
TCGACTA5050.011.76194312
CGAATGT16300.011.7420393
GTCGGTT18750.011.6251751
CGCCCTA3155.2714313E-911.183431
AATCCGA15050.010.52452924
CTCGTAT7150.010.1618671
AAATCCG15750.010.05677123
TCGGTTT22100.010.0540492
TAAGGCG18850.09.9200599
CCCGTAT8850.09.7025721
CGGCTAT5700.09.6567451
ATCCGAA15900.09.54681825
CGACTAG6550.09.40423313
CGTTTCG2353.196432E-49.3614683
TACCGCG2353.196816E-49.36135722
ATTCGAC6900.09.24603510
CGCGTAT2150.00146985129.2165761
ACCGGTA10050.09.1936927
Produced by FastQC (version 0.11.2)