FastQCFastQC Report
Sun 24 Apr 2016
G130-M9_GGCTAC_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M9_GGCTAC_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10208916
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG26050.010.7255483
TCATACG11150.09.86510310
CCGGAAT13300.09.7604211
ATACGAT12050.09.31094712
TAGCTCG13850.09.21355522
ACTACGC9100.09.1873721
CGGGTAT7200.09.1676271
GACGATC7500.09.0941972
CGACAAT6300.09.0803171
TCTTCGT49100.08.91607744
CGACGAT5405.311449E-108.5564521
GGGGAAT23700.08.5409791
CCCACTA33400.08.4319392
TAGCCTC49100.08.4244252
CATACGA14150.08.39544611
CTAGCCT52250.08.2113871
GGAAGAT65850.08.1861271
CTCTTCG52450.08.09482943
CGCAAAT5506.728442E-98.0008371
GCACGTT23650.07.99970237
Produced by FastQC (version 0.11.2)