FastQCFastQC Report
Mon 25 Apr 2016
G129-M11_GGCTAC_AC8VLWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M11_GGCTAC_AC8VLWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19890126
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC3804571.9127933126215488TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA455250.042.98261
ACGGCTA450400.042.3908232
ATCGGAA460650.042.3311732
CGTATGC457250.042.18922444
TCGTATG459200.042.09462443
CGGCTAC454050.042.04520433
ACACGTC467800.041.8673613
CTCGTAT453200.041.8326942
ACGTCTG472300.041.5196915
CGTCTGA473150.041.44045316
TCTCGTA439750.041.1904441
CACACGT475950.041.16892612
CACGTCT477100.041.1480814
ATCTCGT446350.040.60139540
CACGGCT472100.040.52773731
AGTCACG477450.040.49291228
TCACGGC473500.040.44507630
TCGGAAG486650.040.1690183
GCACACG487650.040.1270411
GTCACGG482250.039.77510529
Produced by FastQC (version 0.11.2)