Basic Statistics
Measure | Value |
---|---|
Filename | G129-M1_ATCACG_AC8VLWANXX_L007_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 17441727 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC | 861361 | 4.938507522792897 | TruSeq Adapter, Index 1 (100% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC | 19250 | 0.11036751119886236 | TruSeq Adapter, Index 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 102740 | 0.0 | 43.542942 | 1 |
ATCGGAA | 103495 | 0.0 | 43.094093 | 2 |
CGTATGC | 103175 | 0.0 | 42.880974 | 44 |
ACACGTC | 104680 | 0.0 | 42.872902 | 13 |
ACGTCTG | 105055 | 0.0 | 42.803627 | 15 |
CGTCTGA | 104645 | 0.0 | 42.784233 | 16 |
CTCGTAT | 100290 | 0.0 | 42.593258 | 42 |
TCGTATG | 104145 | 0.0 | 42.574833 | 43 |
TCGGAAG | 104785 | 0.0 | 42.563564 | 3 |
CACACGT | 105490 | 0.0 | 42.562473 | 12 |
CACGTCT | 105735 | 0.0 | 42.513783 | 14 |
ATCACGA | 102455 | 0.0 | 42.39771 | 34 |
ACGATCT | 94225 | 0.0 | 42.39671 | 37 |
TCACGAT | 96800 | 0.0 | 42.386314 | 35 |
CGGAAGA | 105060 | 0.0 | 42.252983 | 4 |
CACGATC | 94385 | 0.0 | 42.224373 | 36 |
TCTCGTA | 96015 | 0.0 | 42.19095 | 41 |
GCACACG | 106260 | 0.0 | 42.146393 | 11 |
CGATCTC | 95895 | 0.0 | 41.953934 | 38 |
GATCTCG | 96125 | 0.0 | 41.935947 | 39 |