FastQCFastQC Report
Mon 25 Apr 2016
G129-M1_ATCACG_AC8VLWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M1_ATCACG_AC8VLWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17441727
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC8613614.938507522792897TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC192500.11036751119886236TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1027400.043.5429421
ATCGGAA1034950.043.0940932
CGTATGC1031750.042.88097444
ACACGTC1046800.042.87290213
ACGTCTG1050550.042.80362715
CGTCTGA1046450.042.78423316
CTCGTAT1002900.042.59325842
TCGTATG1041450.042.57483343
TCGGAAG1047850.042.5635643
CACACGT1054900.042.56247312
CACGTCT1057350.042.51378314
ATCACGA1024550.042.3977134
ACGATCT942250.042.3967137
TCACGAT968000.042.38631435
CGGAAGA1050600.042.2529834
CACGATC943850.042.22437336
TCTCGTA960150.042.1909541
GCACACG1062600.042.14639311
CGATCTC958950.041.95393438
GATCTCG961250.041.93594739