FastQCFastQC Report
Mon 25 Apr 2016
G129-M2_CGATGT_AC8VLWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M2_CGATGT_AC8VLWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14919330
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4344842.91222192953705Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT529050.043.02832811
CGTCGTG532850.042.7707312
GATCGGA521500.042.7494051
TCGTGTA524450.042.67992414
CGTGTAG524550.042.66730515
GTCGTGT538600.042.6163613
AGCGTCG527850.042.5390910
TCTCGGT528700.042.51675436
GTAGATC525750.042.50929631
ATCGGAA521550.042.4568252
GTAGGGA517250.042.44043418
AGAGCGT532300.042.3951958
GATCTCG526000.042.3174734
TGTAGGG536100.042.31461317
GTGGTCG526450.042.21330341
GAGCGTC531450.042.1965839
TGGTCGC521000.042.09358242
GTCGCCG521450.042.08975244
ATCTCGG530600.042.0832835
GTGTAGG539550.042.03589216