Basic Statistics
Measure | Value |
---|---|
Filename | G129-M2_CGATGT_AC8VLWANXX_L007_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14919330 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 434484 | 2.91222192953705 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCGTCGT | 52905 | 0.0 | 43.028328 | 11 |
CGTCGTG | 53285 | 0.0 | 42.77073 | 12 |
GATCGGA | 52150 | 0.0 | 42.749405 | 1 |
TCGTGTA | 52445 | 0.0 | 42.679924 | 14 |
CGTGTAG | 52455 | 0.0 | 42.667305 | 15 |
GTCGTGT | 53860 | 0.0 | 42.61636 | 13 |
AGCGTCG | 52785 | 0.0 | 42.53909 | 10 |
TCTCGGT | 52870 | 0.0 | 42.516754 | 36 |
GTAGATC | 52575 | 0.0 | 42.509296 | 31 |
ATCGGAA | 52155 | 0.0 | 42.456825 | 2 |
GTAGGGA | 51725 | 0.0 | 42.440434 | 18 |
AGAGCGT | 53230 | 0.0 | 42.395195 | 8 |
GATCTCG | 52600 | 0.0 | 42.31747 | 34 |
TGTAGGG | 53610 | 0.0 | 42.314613 | 17 |
GTGGTCG | 52645 | 0.0 | 42.213303 | 41 |
GAGCGTC | 53145 | 0.0 | 42.196583 | 9 |
TGGTCGC | 52100 | 0.0 | 42.093582 | 42 |
GTCGCCG | 52145 | 0.0 | 42.089752 | 44 |
ATCTCGG | 53060 | 0.0 | 42.08328 | 35 |
GTGTAGG | 53955 | 0.0 | 42.035892 | 16 |