FastQCFastQC Report
Mon 25 Apr 2016
G129-M4_TGACCA_AC8VLWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M4_TGACCA_AC8VLWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15829129
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC7437854.698837188072698TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA873200.043.5601771
ATCGGAA875550.043.253622
ACACGTC887950.042.8106313
CGTATGC879750.042.78226544
CGTCTGA889600.042.7089716
ACGTCTG893250.042.66744215
CACACGT895100.042.512912
CTCGTAT855900.042.4870742
TCGTATG888000.042.4721343
CACGTCT897000.042.471914
TCTCGTA820950.042.2714941
AATCTCG813700.042.25565739
CGGAAGA894000.042.112444
TCGGAAG899500.042.089733
ATCTCGT823100.042.0570940
GCACACG902300.042.054211
GACCAAT832300.042.0397235
ACCAATC814300.041.82892236
ACTGACC894200.041.6014132
TGACCAA891800.041.32647734