Basic Statistics
Measure | Value |
---|---|
Filename | G129-M4_TGACCA_AC8VLWANXX_L007_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 15829129 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC | 743785 | 4.698837188072698 | TruSeq Adapter, Index 4 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 87320 | 0.0 | 43.560177 | 1 |
ATCGGAA | 87555 | 0.0 | 43.25362 | 2 |
ACACGTC | 88795 | 0.0 | 42.81063 | 13 |
CGTATGC | 87975 | 0.0 | 42.782265 | 44 |
CGTCTGA | 88960 | 0.0 | 42.70897 | 16 |
ACGTCTG | 89325 | 0.0 | 42.667442 | 15 |
CACACGT | 89510 | 0.0 | 42.5129 | 12 |
CTCGTAT | 85590 | 0.0 | 42.48707 | 42 |
TCGTATG | 88800 | 0.0 | 42.47213 | 43 |
CACGTCT | 89700 | 0.0 | 42.4719 | 14 |
TCTCGTA | 82095 | 0.0 | 42.27149 | 41 |
AATCTCG | 81370 | 0.0 | 42.255657 | 39 |
CGGAAGA | 89400 | 0.0 | 42.11244 | 4 |
TCGGAAG | 89950 | 0.0 | 42.08973 | 3 |
ATCTCGT | 82310 | 0.0 | 42.05709 | 40 |
GCACACG | 90230 | 0.0 | 42.0542 | 11 |
GACCAAT | 83230 | 0.0 | 42.03972 | 35 |
ACCAATC | 81430 | 0.0 | 41.828922 | 36 |
ACTGACC | 89420 | 0.0 | 41.60141 | 32 |
TGACCAA | 89180 | 0.0 | 41.326477 | 34 |