Basic Statistics
Measure | Value |
---|---|
Filename | G129-M4_TGACCA_AC8VLWANXX_L007_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 15829129 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 708899 | 4.478446034522809 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCGTCGT | 84990 | 0.0 | 43.42729 | 11 |
CGTCGTG | 85155 | 0.0 | 43.368706 | 12 |
GATCGGA | 83580 | 0.0 | 43.2826 | 1 |
TCGTGTA | 84285 | 0.0 | 43.205765 | 14 |
AGCGTCG | 84650 | 0.0 | 43.14762 | 10 |
CGTGTAG | 84500 | 0.0 | 43.075005 | 15 |
GTCGTGT | 86555 | 0.0 | 43.012882 | 13 |
AGAGCGT | 85425 | 0.0 | 42.901337 | 8 |
TCTCGGT | 84915 | 0.0 | 42.89952 | 36 |
GATCTCG | 84155 | 0.0 | 42.87969 | 34 |
GAGCGTC | 85075 | 0.0 | 42.86383 | 9 |
GTAGATC | 84750 | 0.0 | 42.861855 | 31 |
GTAGGGA | 82785 | 0.0 | 42.85171 | 18 |
ATCGGAA | 84040 | 0.0 | 42.766087 | 2 |
TGTAGGG | 86060 | 0.0 | 42.74448 | 17 |
ATCTCGG | 84690 | 0.0 | 42.73157 | 35 |
GTGTAGG | 86220 | 0.0 | 42.667305 | 16 |
AAGAGCG | 84565 | 0.0 | 42.648033 | 7 |
GTCGCCG | 83340 | 0.0 | 42.61261 | 44 |
GGTCGCC | 83130 | 0.0 | 42.580624 | 43 |