FastQCFastQC Report
Mon 25 Apr 2016
G129-M4_TGACCA_AC8VLWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M4_TGACCA_AC8VLWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15829129
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7088994.478446034522809Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT849900.043.4272911
CGTCGTG851550.043.36870612
GATCGGA835800.043.28261
TCGTGTA842850.043.20576514
AGCGTCG846500.043.1476210
CGTGTAG845000.043.07500515
GTCGTGT865550.043.01288213
AGAGCGT854250.042.9013378
TCTCGGT849150.042.8995236
GATCTCG841550.042.8796934
GAGCGTC850750.042.863839
GTAGATC847500.042.86185531
GTAGGGA827850.042.8517118
ATCGGAA840400.042.7660872
TGTAGGG860600.042.7444817
ATCTCGG846900.042.7315735
GTGTAGG862200.042.66730516
AAGAGCG845650.042.6480337
GTCGCCG833400.042.6126144
GGTCGCC831300.042.58062443