FastQCFastQC Report
Mon 25 Apr 2016
G129-M7_CAGATC_AC8VLWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M7_CAGATC_AC8VLWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15547911
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4134402.6591353655163066Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT504850.043.1432211
CGTCGTG507850.042.90960712
TCGTGTA501600.042.79957214
GATCGGA499350.042.7560421
CGTGTAG501500.042.7198315
AGCGTCG505700.042.60972610
GATCTCG499750.042.44490434
GTCGTGT517250.042.43176713
TCTCGGT506350.042.42944336
GTAGGGA493950.042.35821518
GTAGATC504000.042.3011431
ATCGGAA502550.042.2055322
AGAGCGT512850.042.2051168
GAGCGTC510950.042.154529
GTGGTCG502350.042.10206241
TGTAGGG515300.042.0547117
ATCTCGG506100.041.9907335
TGGTCGC497900.041.92618642
GTGTAGG517750.041.88517816
AAGAGCG509850.041.7715077
Produced by FastQC (version 0.11.2)