FastQCFastQC Report
Mon 25 Apr 2016
G129-M8_ACTTGA_AC8VLWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M8_ACTTGA_AC8VLWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19494518
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC16844888.640829180798418TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGC312150.1601219378699181TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAACTCGTATGC259400.13306304880171954TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1969900.043.7183651
ATCGGAA1974300.043.4304352
CGTATGC1972700.043.12532844
ACACGTC2002000.043.06434613
CGTCTGA2002700.043.0317216
ACGTCTG2009200.042.9954315
TCGGAAG1993300.042.992183
CACACGT2008850.042.91640512
CACGTCT2014200.042.8952514
TCGTATG1991350.042.8108443
CGGAAGA1993700.042.771374
CTCGTAT1936150.042.73377642
GCACACG2014850.042.66413511
TCTCGTA1851100.042.6408141
AATCTCG1837650.042.4775939
ATCTCGT1857550.042.4124240
TTGAATC1840350.042.2952936
GAACTCC2030500.042.17929521
CTGAACT2041100.042.11652419
GTCTGAA2046050.042.1103217
Produced by FastQC (version 0.11.2)