Basic Statistics
Measure | Value |
---|---|
Filename | G129-M9_GATCAG_AC8VLWANXX_L007_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 20794591 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC | 1070860 | 5.149704555381733 | TruSeq Adapter, Index 9 (100% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC | 22044 | 0.10600833649481252 | TruSeq Adapter, Index 9 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGC | 21998 | 0.10578712512306686 | TruSeq Adapter, Index 9 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 128105 | 0.0 | 43.56541 | 1 |
ATCGGAA | 128675 | 0.0 | 43.240017 | 2 |
CGTATGC | 128765 | 0.0 | 43.018696 | 44 |
ACACGTC | 130800 | 0.0 | 42.744194 | 13 |
TCGTATG | 130060 | 0.0 | 42.668034 | 43 |
TCGGAAG | 130265 | 0.0 | 42.644684 | 3 |
CTCGTAT | 125630 | 0.0 | 42.64316 | 42 |
CGTCTGA | 130910 | 0.0 | 42.624256 | 16 |
ACGTCTG | 131370 | 0.0 | 42.59055 | 15 |
CGATCAG | 128865 | 0.0 | 42.522236 | 33 |
CACACGT | 131630 | 0.0 | 42.493053 | 12 |
ACGATCA | 129000 | 0.0 | 42.477737 | 32 |
TCACGAT | 129645 | 0.0 | 42.471085 | 30 |
CACGTCT | 131910 | 0.0 | 42.44955 | 14 |
GTCACGA | 129925 | 0.0 | 42.425274 | 29 |
TCTCGTA | 118950 | 0.0 | 42.351463 | 41 |
CACGATC | 129720 | 0.0 | 42.297283 | 31 |
CGGAAGA | 130825 | 0.0 | 42.28033 | 4 |
AGTCACG | 131280 | 0.0 | 42.20524 | 28 |
ATCTCGT | 119235 | 0.0 | 42.165455 | 40 |