FastQCFastQC Report
Mon 25 Apr 2016
G129-M9_GATCAG_AC8VLWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M9_GATCAG_AC8VLWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20794591
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC10708605.149704555381733TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC220440.10600833649481252TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGC219980.10578712512306686TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1281050.043.565411
ATCGGAA1286750.043.2400172
CGTATGC1287650.043.01869644
ACACGTC1308000.042.74419413
TCGTATG1300600.042.66803443
TCGGAAG1302650.042.6446843
CTCGTAT1256300.042.6431642
CGTCTGA1309100.042.62425616
ACGTCTG1313700.042.5905515
CGATCAG1288650.042.52223633
CACACGT1316300.042.49305312
ACGATCA1290000.042.47773732
TCACGAT1296450.042.47108530
CACGTCT1319100.042.4495514
GTCACGA1299250.042.42527429
TCTCGTA1189500.042.35146341
CACGATC1297200.042.29728331
CGGAAGA1308250.042.280334
AGTCACG1312800.042.2052428
ATCTCGT1192350.042.16545540