Basic Statistics
Measure | Value |
---|---|
Filename | G129-M9_GATCAG_AC8VLWANXX_L007_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 20794591 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 1012091 | 4.8670877922051945 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCGTG | 124120 | 0.0 | 43.302414 | 12 |
GCGTCGT | 124130 | 0.0 | 43.295696 | 11 |
GATCGGA | 122015 | 0.0 | 43.217655 | 1 |
TCGTGTA | 122785 | 0.0 | 43.20707 | 14 |
AGCGTCG | 123035 | 0.0 | 43.19152 | 10 |
CGTGTAG | 122915 | 0.0 | 43.16475 | 15 |
ATCGGAA | 121445 | 0.0 | 43.084343 | 2 |
GTCGTGT | 125890 | 0.0 | 43.044827 | 13 |
GATCTCG | 122195 | 0.0 | 42.98188 | 34 |
TCTCGGT | 123420 | 0.0 | 42.973637 | 36 |
GTCGCCG | 120315 | 0.0 | 42.94297 | 44 |
GTAGGGA | 120875 | 0.0 | 42.941616 | 18 |
AGAGCGT | 124155 | 0.0 | 42.91633 | 8 |
GAGCGTC | 123720 | 0.0 | 42.882683 | 9 |
GTAGATC | 123215 | 0.0 | 42.879604 | 31 |
TGTAGGG | 125425 | 0.0 | 42.839592 | 17 |
ATCTCGG | 122825 | 0.0 | 42.813873 | 35 |
TGGTCGC | 120740 | 0.0 | 42.760498 | 42 |
GTGGTCG | 122300 | 0.0 | 42.758366 | 41 |
AAGAGCG | 122760 | 0.0 | 42.638607 | 7 |