FastQCFastQC Report
Mon 25 Apr 2016
G129-M9_GATCAG_AC8VLWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M9_GATCAG_AC8VLWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20794591
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG10120914.8670877922051945Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG1241200.043.30241412
GCGTCGT1241300.043.29569611
GATCGGA1220150.043.2176551
TCGTGTA1227850.043.2070714
AGCGTCG1230350.043.1915210
CGTGTAG1229150.043.1647515
ATCGGAA1214450.043.0843432
GTCGTGT1258900.043.04482713
GATCTCG1221950.042.9818834
TCTCGGT1234200.042.97363736
GTCGCCG1203150.042.9429744
GTAGGGA1208750.042.94161618
AGAGCGT1241550.042.916338
GAGCGTC1237200.042.8826839
GTAGATC1232150.042.87960431
TGTAGGG1254250.042.83959217
ATCTCGG1228250.042.81387335
TGGTCGC1207400.042.76049842
GTGGTCG1223000.042.75836641
AAGAGCG1227600.042.6386077