FastQCFastQC Report
Fri 22 Apr 2016
G130-M1_GCCAAT_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M1_GCCAAT_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12662659
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT20700.030.28851911
CGTCGTG22450.027.82972512
GATCGGA23850.026.3845561
TCGTGTA25950.024.83917614
CGTGTAG25950.024.24574915
GTCGTGT27000.024.11764313
GATCTCG25950.023.06007834
AGCGTCG27300.023.046610
GTCGCCG26700.022.82314344
AGAGCGT28550.022.4231828
GTAGATC26800.022.41122631
GAGCGTC28550.022.3462149
TCTCGGT27700.022.23807536
GTAGGGA28200.022.15653818
ATCGGAA29200.021.1732252
GGTCGCC29100.021.0161743
GTGTAGG32400.019.55484616
ATCTCGG31850.019.27164835
GTGGTCG32900.019.12362541
AAGAGCG33350.019.0639277
Produced by FastQC (version 0.11.2)