FastQCFastQC Report
Fri 22 Apr 2016
G130-M3_ACTTGA_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M3_ACTTGA_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12794606
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA19650.028.7947981
TCGTATG21600.026.6868543
CGTATGC21300.026.65058944
ATCGGAA23100.024.2848572
ACACGTC24000.024.10729213
ACGTCTG23900.024.02406715
CTCGTAT24450.023.39577742
CGTCTGA25850.022.72242416
AATCTCG26350.020.2896939
TCTCGTA27400.019.51186241
CACGTCT30200.019.30380214
CACACGT31450.018.5365612
CGGAAGA33250.017.5992414
TCGGAAG35850.016.2002623
ATCTCGT33500.015.95920540
GCACACG38600.015.27395111
TTGAATC42550.012.77165436
GAACTCC50850.011.81069921
GAATCTC47100.011.63129838
GTCGGTT13200.011.0081291
Produced by FastQC (version 0.11.2)