FastQCFastQC Report
Fri 22 Apr 2016
G130-M6_CTTGTA_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M6_CTTGTA_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13096276
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC188440.14388823204397952TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA27800.032.1554761
CGTATGC29400.030.6837444
TCGTATG30200.029.86955843
ATCGGAA31400.028.4446242
CTCGTAT32050.027.59539642
ACGTCTG33750.027.5068515
ACACGTC33900.027.25534813
AATCTCG32850.026.52243439
CGTCTGA36150.025.31553616
CACGTCT39350.023.7040914
TCTCGTA37700.023.34294341
CACACGT39700.023.27345812
CGGAAGA41800.022.1568474
TCGGAAG45100.020.5356923
GCACACG46300.020.00337411
ATCTCGT44400.019.92033640
GTAATCT48350.018.24734937
AGTCACC56050.016.95548628
TAATCTC52200.016.85943638
TGTAATC55500.016.05513436
Produced by FastQC (version 0.11.2)