FastQCFastQC Report
Fri 22 Apr 2016
G130-M6_CTTGTA_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M6_CTTGTA_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13096276
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG175270.13383193817845623Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT28000.031.97677611
CGTCGTG28300.031.09449212
TCGTGTA31250.028.8631514
AGCGTCG31900.028.20531310
GATCTCG31000.028.1744434
GATCGGA31700.027.4161071
GTAGATC33550.026.4932531
GTCGTGT33900.026.41220313
TCTCGGT33200.026.30695736
CGTGTAG33750.026.1384915
ATCTCGG34600.025.24270635
GTCGCCG34950.025.052144
TGGTCGC36400.024.355542
GAGCGTC37250.024.2138679
AGAGCGT38100.023.9045358
GGTCGCC36900.023.84662643
GTAGGGA36850.023.8216518
ATCGGAA36100.023.769882
GTGGTCG39500.022.38836141
TGTAGGG40150.022.35687317
Produced by FastQC (version 0.11.2)