FastQCFastQC Report
Fri 22 Apr 2016
G130-M7_AGTCAA_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M7_AGTCAA_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12384150
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255900.2066350940516709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA14450.030.3198971
ATCGGAA18700.023.8815172
CTCGTAT19150.021.82929244
CGTCTGA20650.021.41329416
ACACGTC20550.021.41044213
ACGTCTG21150.020.69903815
TCTCGTA22900.019.5033943
AATCTCG21750.019.11765341
CACACGT25650.017.75376512
CACGTCT25800.017.13893314
CGGAAGA31950.015.1481394
TCGGAAG33350.014.4462713
ATCTCGT30000.014.22731742
GCACACG33750.013.88395711
AGTCAAC32450.013.62686234
TCGACCG4750.010.65225827
GTCGGTA7900.010.3113871
GTCCGGA8500.09.842541
GTCTGAA49050.09.82228617
CAACAAT47100.09.76249637
Produced by FastQC (version 0.11.2)