FastQCFastQC Report
Fri 22 Apr 2016
G130-M8_AGTTCC_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M8_AGTTCC_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5375007
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260650.48492960102191496No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA189300.3521855878513275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG5300.026.97996712
GCGTCGT5350.025.49422811
CGTGTAG6400.020.28018615
AGCGTCG7150.019.99895110
GTCGCCG6550.019.47989544
GTCGTGT7350.018.85615313
TCTCGGT7500.018.47954636
GATCTCG7200.018.33305434
GAGCGTC8100.017.1106919
TCGTGTA8000.017.04910714
GATCGGA7950.016.6053751
AGAGCGT9750.015.3433568
ATCTCGG9250.015.2213935
GTGGTCG8700.014.91875841
GTAGGGA9000.014.91213118
GTAGATC8950.014.74906431
CTCGGTG9450.014.6661737
GGTCGCC9500.014.35714243
CGGTACG950.006240695713.89568818
CGACGGT950.00624569813.89400843
Produced by FastQC (version 0.11.2)