FastQCFastQC Report
Tue 9 Aug 2016
G132-M1-B_ATCACG_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M1-B_ATCACG_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14104575
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA24050.028.5774771
CGTATGC23700.027.94138144
TCGTATG24650.027.57844243
ATCGGAA26400.025.581752
ATCACGA27250.024.3011434
ACACGTC29950.022.69642813
CTCGTAT29200.022.22622942
ACGATCT28250.022.03859137
TCACGAT29850.021.59506635
ACGTCTG31500.021.57961815
CGTCTGA33650.020.26620716
TCTCGTA34750.018.36007541
CACACGT37750.018.29820612
CACGATC34050.018.22062536
CGGAAGA38900.017.8138084
CATCACG38100.017.6694533
CACGTCT39600.017.44336714
GATCTCG37800.016.99420239
ATCTCGT38600.016.699140
CGATCTC39850.016.34061838
Produced by FastQC (version 0.11.2)