FastQCFastQC Report
Tue 9 Aug 2016
G132-M1-B_ATCACG_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M1-B_ATCACG_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14104575
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT22250.029.56289711
CGTCGTG25100.026.20615412
TCGTGTA25900.025.65078514
CGTGTAG25800.025.3237915
AGCGTCG27650.023.7894810
GTCGTGT29400.023.49560413
GATCTCG28200.023.48160734
GATCGGA29400.022.7472551
GTAGGGA30150.021.96274218
TCTCGGT30750.021.53435136
GTAGATC31600.021.51144231
ATCTCGG31000.021.5026235
GTGTAGG32800.020.38880216
ATCGGAA33000.019.999082
AGAGCGT33550.019.802658
TGTAGGG33750.019.74971417
GAGCGTC34400.019.3133419
GGTCGCC33750.019.172443
TGGTCGC34150.019.010842
GTCGCCG34600.018.51109744
Produced by FastQC (version 0.11.2)