FastQCFastQC Report
Tue 9 Aug 2016
G132-M10-B_GTGAAA_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M10-B_GTGAAA_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12760577
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1525371.195376980210221No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT670190.5252035233203013TruSeq Adapter, Index 19 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA421870.3306041725229196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA81250.041.15811
CGTGAAA82650.039.68707333
AACGATC75900.039.53562538
TGAAACG83550.039.20797735
GAAACGA83750.038.9304636
ATCGGAA85750.038.7894172
AAACGAT80150.038.50985337
ACGTGAA85900.038.3137332
ACACGTC87450.038.21145213
ACGTCTG88450.037.7794415
CTCGTAT85100.037.43317844
CGTCTGA89050.037.35196316
GTCACGT88950.037.22360629
ACGATCT81300.037.12699539
CACGTGA88550.036.99495731
AGTCACG90600.036.56997728
CACACGT93050.036.12455712
CACGTCT94950.035.30901314
TCTCGTA86500.035.27575343
CGGAAGA95250.034.8513684
Produced by FastQC (version 0.11.2)