FastQCFastQC Report
Tue 9 Aug 2016
G132-M12-B_GTTTCG_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M12-B_GTTTCG_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12019161
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1116180.9286671507270765No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT389260.3238661999785176TruSeq Adapter, Index 21 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA132330.11009919910383095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG49050.039.96328433
GATCGGA53850.037.024041
TCGGAAT52300.035.20810337
ATCGGAA56550.035.127912
ACACGTC57550.034.5939713
CGGAATC52150.034.422938
CTCGTAT55250.034.363544
GTTTCGG57500.034.16694334
ACGTTTC57400.034.1114832
ACGTCTG59600.033.84700415
AATCTCG53200.033.8273341
AGTCACG59500.033.3139928
CGTCTGA60450.033.04355216
GTCACGT60200.032.7804429
TTTCGGA61100.032.2625235
TTCGGAA60350.032.0072836
CACGTCT64050.031.32368714
TCTCGTA59050.030.99705743
CACACGT64700.030.7369912
CGGAAGA67200.029.9208624
Produced by FastQC (version 0.11.2)