FastQCFastQC Report
Tue 9 Aug 2016
G132-M12-B_GTTTCG_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M12-B_GTTTCG_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12019161
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG361580.30083630629459074Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA174520.14520148286556775No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147420.12265415198282144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT50700.037.61984611
CGTCGTG51900.036.83479712
TCGTGTA53100.036.04320514
CGTGTAG53300.035.70129415
GTCGTGT54350.035.7005513
GATCTCG52800.035.5402934
ATCTCGG53650.034.9772135
GATCGGA55450.034.0796931
GTAGATC55100.033.97634531
TCTCGGT56500.033.7969236
GTCGCCG54750.033.48840744
GTAGGGA56900.033.24989318
AGCGTCG57850.032.74203510
GGTCGCC58000.032.10350843
AGAGCGT59700.032.0959138
GAGCGTC60650.031.411819
TGTAGGG61900.031.3453417
GTGGTCG58950.031.24505441
TAGATCT60450.031.11529432
TGGTCGC60700.030.85449642
Produced by FastQC (version 0.11.2)