FastQCFastQC Report
Tue 9 Aug 2016
G132-M14-B_GAGTGG_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M14-B_GAGTGG_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14093131
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT431250.3060001358108429TruSeq Adapter, Index 7 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234610.1664711695364217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA56950.036.7169231
ACGAGTG57150.036.2630132
CGAGTGG59550.034.87516833
ATCGGAA60050.034.838892
TATCTCG56050.034.81541
TCACGAG60800.033.9774830
ACACGTC62050.033.7159613
CACGAGT61550.033.67128831
ACGTCTG62500.033.578815
CTCGTAT60450.033.33607544
AGTCACG62750.033.1656228
CGTCTGA65100.032.03495816
GTCACGA65350.031.74510629
CACACGT67950.030.853212
CACGTCT68650.030.7308714
TCTCGTA66750.029.596543
CGGAAGA72050.029.1585664
GTGGATA70350.029.14644636
GGATATC68400.028.52814738
TCGGAAG74350.028.5228483
Produced by FastQC (version 0.11.2)