FastQCFastQC Report
Tue 9 Aug 2016
G132-M14-B_GAGTGG_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M14-B_GAGTGG_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14093131
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG392180.2782774104632959Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT52900.037.88486511
CGTCGTG56350.035.56538412
CGTGTAG56800.034.89544715
TCGTGTA58050.034.59890714
GATCGGA58400.034.091051
GATCTCG58650.033.87095334
AGCGTCG59250.033.60209710
GTCGTGT61100.033.34055713
TCTCGGT61100.032.90884836
ATCTCGG61950.032.17322535
GTAGATC62050.032.01455731
GTCGCCG61200.031.9008244
GTAGGGA60650.031.8468718
GGTCGCC61950.031.37160143
AGAGCGT64250.031.3295468
TGTAGGG64750.030.84880617
ATCGGAA63650.030.7952632
GAGCGTC65750.030.414059
GTGTAGG66850.030.0442716
TAGATCT68400.029.49292432
Produced by FastQC (version 0.11.2)