FastQCFastQC Report
Tue 9 Aug 2016
G132-M15-B_ACTGAT_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M15-B_ACTGAT_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13961466
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT493900.3537594117981593TruSeq Adapter, Index 25 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246680.17668631646562044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA63800.038.28991
TATCTCG63250.036.17440841
ATCGGAA68000.035.9096832
ACGTCTG70700.034.94281415
CTCGTAT68300.034.65883644
ACACGTC71850.034.26106313
CGTCTGA72400.034.09194616
CACACGT77450.031.9826512
CACGTCT78200.031.76030714
GATATAT73350.031.70277437
TCTCGTA73450.031.59959443
ATATCTC74700.030.71729740
CGGAAGA79800.030.714
GCACACG82750.029.80129211
ATATATC77700.029.67233738
ATCTCGT78700.029.26787842
TCGGAAG83700.029.2265033
TGATATA85550.028.28793736
TATATCT83700.027.86119539
ACTGATA89000.027.63564134
Produced by FastQC (version 0.11.2)