FastQCFastQC Report
Tue 9 Aug 2016
G132-M16-B_ATTCCT_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M16-B_ATTCCT_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13635953
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT826140.6058542442908097TruSeq Adapter, Index 27 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT781510.5731245920252146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA104250.039.3442041
TATCTCG97550.038.67801741
ATCGGAA105600.038.643562
CTCGTAT102900.038.16325444
ACACGTC108850.037.87374513
CGTCTGA108800.037.83049416
ACGTCTG109250.037.67466715
CACGTCT114050.036.30123514
CACACGT114050.036.1083512
TCTCGTA107300.035.42949743
CGGAAGA115850.035.1295664
GCACACG118750.034.58659411
TCGGAAG119800.034.2100033
ATCTCGT116200.032.56441542
TTATCTC121650.031.30432340
GAACTCC137600.029.97654221
GTCTGAA139300.029.8632817
CTGAACT139900.029.67230419
AGAGCAC137400.029.667838
TCTGAAC141400.029.41976418
Produced by FastQC (version 0.11.2)