FastQCFastQC Report
Tue 9 Aug 2016
G132-M16-B_ATTCCT_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M16-B_ATTCCT_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13635953
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG787090.5772167152526853Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT99500.039.82002611
CGTCGTG101050.039.2745412
CGTGTAG101800.038.7889915
GATCTCG100750.038.51735734
GATCGGA102250.038.382751
TCGTGTA104400.037.9916914
ATCTCGG103050.037.76441635
GTAGGGA102900.037.6703718
GTCGTGT106450.037.65380513
GTAGATC104350.037.4620531
TCTCGGT105250.037.35127336
GTCGCCG102450.037.18734444
AGCGTCG106000.037.15007410
GTGTAGG108650.036.74842516
ATCGGAA107150.036.4221842
AGAGCGT109250.036.1657458
GAGCGTC109250.036.0851979
TAGATCT108700.036.04409432
GTGGTCG106900.036.00463541
GGTCGCC107400.035.6567943
Produced by FastQC (version 0.11.2)