FastQCFastQC Report
Tue 9 Aug 2016
G132-M2-B_CGATGT_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M2-B_CGATGT_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17957441
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339630.18913051141306825No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC314690.17524211829514016TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA45450.034.171561
TCGTATG46500.033.1175443
CGTATGC48400.031.6813744
TCACCGA49500.031.28923430
TATCTCG45700.031.19396239
ATCGGAA50950.030.4401662
CTCGTAT49800.030.30454442
CGATGTA52200.029.41704634
CCGATGT53350.028.94788433
ACACGTC55100.028.22733713
CGTCTGA55350.028.06009516
ACGTCTG55250.027.95161415
CACCGAT57800.027.0246731
ACCGATG58750.026.36225732
GTCACCG59500.026.17829
CACACGT62150.025.23773812
CACGTCT61850.025.21788214
TCTCGTA57950.024.78991941
CGGAAGA65550.023.8951284
ATCTCGT61300.023.39908240
Produced by FastQC (version 0.11.2)