FastQCFastQC Report
Tue 9 Aug 2016
G132-M3-B_GCCAAT_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M3-B_GCCAAT_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12518697
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357310.2854210785675218No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC146010.11663354420991259TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA23150.032.447761
CGCCAAT24150.030.51721433
TATCTCG24450.030.14264939
ACGCCAA25600.029.39060832
ATCGGAA25600.029.3029142
CGTATGC25400.029.18930844
TCGTATG26000.028.76955843
GTCACGC26550.028.17346829
CGTCTGA28350.027.23644616
ACGTCTG28150.027.11736315
AGTCACG28400.026.95794528
CTCGTAT28000.026.7145942
ACACGTC30100.025.36059213
TCACGCC30300.024.75945730
CACACGT32850.023.1705912
TCTCGTA32250.022.92137541
CACGCCA33500.022.7230131
CACGTCT33900.022.5178114
CGGAAGA35400.021.9986694
GCACACG37500.020.70807811
Produced by FastQC (version 0.11.2)