FastQCFastQC Report
Tue 9 Aug 2016
G132-M4-B_CAGATC_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M4-B_CAGATC_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15187612
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT582710.3836745368527982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC356270.23457934005688322TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA51350.036.6370431
CGTATGC54500.034.4731244
ATCGGAA55300.033.813842
TCGTATG55700.033.7699343
CTCGTAT56200.032.64721342
ACACGTC58700.032.2675313
CGTCTGA60650.031.26634416
ACGTCTG60750.031.14245215
CACGTCT64400.029.44571114
CACACGT65200.029.11815512
TCTCGTA60900.029.00807441
CGGAAGA68650.027.686874
GCACACG72050.026.31927711
TCGGAAG73950.025.7620473
ATCTCGT71950.024.7052840
CATCTCG72650.024.40667739
AGTCACC85600.022.20521428
ACCAGAT87100.022.02530132
TCTGAAC95100.020.26395418
GTCTGAA97300.020.099717
Produced by FastQC (version 0.11.2)