FastQCFastQC Report
Tue 9 Aug 2016
G132-M4-B_CAGATC_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M4-B_CAGATC_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15187612
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG333830.21980414037440515Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG51250.035.4135312
GCGTCGT51650.035.18198411
GATCGGA53600.033.778461
GTCGTGT55300.033.2176613
CGTGTAG54350.033.06871415
TCGTGTA54650.032.9274414
GATCTCG55650.032.5342634
TCTCGGT58500.031.4381336
AGCGTCG57550.031.3841110
ATCTCGG58300.031.28183735
GTAGATC58900.030.77561631
AGAGCGT59600.030.6737428
GTAGGGA58150.030.60659818
GGTCGCC59500.030.22039643
GTCGCCG59850.029.56668944
GAGCGTC62250.029.4739749
ATCGGAA61900.029.1070422
GTGTAGG63400.028.97290416
GTGGTCG62750.028.61616341
TGGTCGC63450.028.5447442
Produced by FastQC (version 0.11.2)