FastQCFastQC Report
Tue 9 Aug 2016
G132-M8-B_CCGTCC_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M8-B_CCGTCC_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15174156
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG176480.11630300887904407Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT26050.033.9492411
CGTCGTG28800.030.86023112
TCGTGTA32500.027.68427314
GATCGGA32850.027.3228821
CGTGTAG32800.027.02864815
AGCGTCG33150.026.81087710
GTCGTGT34950.026.24783313
GATCTCG34000.026.07581534
TCTCGGT35600.025.08925836
GTAGGGA34800.024.9703918
ATCTCGG35700.024.8341135
GTCGCCG36300.024.31307644
GAGCGTC37450.024.261159
GTAGATC37050.024.22579231
GGTCGCC37700.024.05174343
AGAGCGT38900.023.3568158
ATCGGAA38250.023.0630172
TGGTCGC40100.022.93995342
GTGGTCG39950.022.3080541
TGTAGGG41550.021.81319817
Produced by FastQC (version 0.11.2)