FastQCFastQC Report
Tue 9 Aug 2016
G132-M9-B_GTCCGC_AC8WFRANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M9-B_GTCCGC_AC8WFRANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11009321
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990311.8078408286941583No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT321620.2921342742209079TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG41850.040.05687732
GATCGGA44300.037.942211
CGTCCGC45200.037.23407433
GTCCGCA46850.035.82882334
ATCGGAA48100.035.1707082
ACACGTC49150.034.64314313
ACGTCTG50350.033.59902615
CGTCTGA50400.033.56569316
CTCGTAT49350.033.4344144
CGCACAT47950.033.0800537
AGTCACG52550.032.2357328
CACACGT53300.032.15215312
GTCACGT53200.031.67646429
TCACGTC53700.031.46360430
CGGAAGA55000.030.9183734
CACGTCT55450.030.74680514
TCTCGTA51800.030.70620243
CACGTCC55550.030.33710331
TCCGCAC55950.030.15926435
GCACACG57250.029.85694311
Produced by FastQC (version 0.11.2)