FastQCFastQC Report
Tue 9 Aug 2016
G132-M9-B_GTCCGC_AC8WFRANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG132-M9-B_GTCCGC_AC8WFRANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11009321
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG292910.26605637168722757Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA247150.224491592169944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT42500.038.77046211
CGTCGTG44750.036.62430612
CGTGTAG45300.035.64414615
GATCTCG44850.035.36559334
GATCGGA45100.034.9254951
GTCGTGT48000.034.41936513
GTAGGGA46950.033.64332618
ATCTCGG47750.033.3559535
TCTCGGT48050.033.33082636
TCGTGTA48350.033.2136614
AGCGTCG49200.033.1777510
GAGCGTC49800.032.9988949
AGAGCGT49850.032.8775338
GTCGCCG47200.032.6414444
GTAGATC49900.032.00621431
GTGTAGG52550.031.3544716
TGTAGGG53400.030.64940517
GGTCGCC51250.030.44765343
ATCGGAA51850.030.25152
TAGATCT52950.030.24611732
Produced by FastQC (version 0.11.2)