FastQCFastQC Report
Wed 29 Jun 2016
G133_M19.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG133_M19.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12298057
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG3700.024.31865715
CGACGCT3650.023.41919917
ACGACGC3950.021.64052616
ACACGAC5950.015.87832814
GATCGGA5650.015.651061
GACGCTC5700.014.60186118
TCGTATG5900.013.72767945
ATCGGAA6100.013.6446932
CTCGTAT6050.013.38705144
CTACACG7250.013.34112212
TACACGA6950.013.26969413
TCTCGTA6000.013.12364943
CGTCTGA7400.012.15932816
TATCTCG7050.011.48813341
CGCTCTT9150.010.81745920
ACGCTCT8500.010.58606919
ACACGTC8150.010.48788413
ATCTCGT7650.09.99897142
ACGTCTG8800.09.71364515
TCGGAAG9900.09.5428043
Produced by FastQC (version 0.11.2)