FastQCFastQC Report
Mon 6 Mar 2017
h2ak5ac_merge.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameh2ak5ac_merge.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51240765
Sequences flagged as poor quality0
Sequence length36
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA69100.022.93779611
CTCGTAT70800.022.28129612
TCGTATG71400.022.07333613
CGTATGC74150.021.47753314
ATCGGAA86400.020.1844642
GATCGGA86900.020.138461
ATGCCGT77550.020.01491717
AGCTCGT79850.019.9622310
GCCGTCT78800.019.81371719
GAGCTCG83300.019.5131749
TGCCGTC79850.019.34472718
CCGTCTT82350.019.06926220
TATGCCG82250.019.0348516
TCGGAAG95250.018.4346563
CGTCTTC87650.017.88160121
CGGAAGA97650.017.352214
GTATGCC115250.013.97441515
AGAGCTC211700.08.2159788
GAAGAGC221700.07.9131786
GTCTTCT218650.07.890917322
Produced by FastQC (version 0.11.2)