FastQCFastQC Report
Sun 7 Aug 2016
GBM10.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM10.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5458952
Sequences flagged as poor quality0
Sequence length83
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAC7050.021.8273435
GTTACGA7900.020.4523984
TACGACT8550.018.4479356
TGTTACG8000.018.2737463
TATCCGG8550.013.4983659
TTATCCG8350.013.3609568
CTCGCGT3101.820586E-512.4140051
AGGCGTA11550.012.32383258
ATCGAAG24650.011.8611476
ACGACTT13300.011.8592787
TCGTGCG9200.011.70836457
CCGGTAA7300.011.5938018
CCGACAT24150.011.4733161
CGTGCGT9250.011.229188
ACGAGCG5505.2077667E-911.1915773
TAGCATA14650.011.029048
TCACCCG28650.010.7423523
GGCTCGT10400.010.7272074
CGGTAAA7900.010.713269
CCCGTAT5052.1622145E-710.668671
Produced by FastQC (version 0.11.2)