FastQCFastQC Report
Sun 7 Aug 2016
GBM102.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM102.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103973159
Sequences flagged as poor quality0
Sequence length83
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA48251.8189894E-1215.25803244-45
CGTATCG68100.013.8699783
TACGCGT17850.006699089413.747944-45
CGTTATA46752.2664608E-913.12446648-49
TGCGAAA89300.012.82469246-47
TACCGTA86300.012.6130719
CGCATTA56052.0372681E-1012.40638648-49
CCCGTAT110850.012.1286491
GTATCGA80300.012.0250574
CTAGCGT41001.072769E-611.97073244-45
CTAGCGA42101.4763646E-611.65795844-45
TTCGCAT84450.011.62390246-47
TTCGCTA60106.493792E-1011.56950746-47
TTAACGA75251.8189894E-1211.41395444-45
ATTTCGT104100.011.39385344-45
TATCGTA46804.2704778E-711.36111244-45
TTTCGTC112400.011.2802544-45
TAGCGAA54702.3354005E-811.21572244-45
TATTCGT77903.6379788E-1211.02567544-45
ATTTCGC79053.6379788E-1210.86527544-45
Produced by FastQC (version 0.11.2)