FastQCFastQC Report
Sun 7 Aug 2016
GBM110.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM110.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11583822
Sequences flagged as poor quality0
Sequence length83
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT2103.183486E-718.3247051
GTTACGA10850.017.3752384
TTACGAC11850.015.2595625
CGTATCG10500.014.2903093
TACGACT13750.013.9903916
TGTTACG14200.012.7343233
TTATCCG15350.012.2814678
TCACCCG33800.011.9519543
AGTACGC18200.010.93703148-49
TATCGTT11000.010.8425527
GCCGTAT11050.010.7935853
GTATCGT10850.010.6378566
TACGCAC18650.010.63242250-51
CCCGTAT12000.010.5825171
CGCAGTT22650.010.1938751
CCGTATC15650.09.8340542
GTTACGG23550.09.6388157
TACGGAT23600.09.4553719
CACCCGG46300.09.3068234
TATCCGG19850.09.3034349
Produced by FastQC (version 0.11.2)