FastQCFastQC Report
Sun 7 Aug 2016
GBM115.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM115.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83611292
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT108400.011.2878311
CGTATCG79200.010.9602223
GTATCGA85250.09.9108234
CCGTATC126250.09.28963852
ATCGAAG205500.07.6047386
CCGGGAT229600.07.31303831
TATCGAA132550.07.2018895
CGGGGAT244150.07.06693841
CCCGGTT129450.07.00722931
CTCGGAT189600.06.98290731
CCCGGAT240700.06.89561271
CCGGAAT139200.06.8214451
CGGGGTT214000.06.81802371
CCGGTAT130200.06.7889871
CGACCGT58050.06.78232241
CTCGGTT125250.06.6566631
CGCGAAT21200.06.55461451
TCGAAGG241900.06.460327
CTCGAAT139700.06.32731681
CTCGTTT145900.06.2700871
Produced by FastQC (version 0.11.2)