FastQCFastQC Report
Sun 7 Aug 2016
GBM116.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM116.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39608215
Sequences flagged as poor quality0
Sequence length83
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT38400.011.2528141
CGTATCG44250.010.9861893
CCGACAT104800.010.58281
ATCGAAG124300.010.57537656
CGCGTAA35350.010.4920171
TTCGCGC39700.09.797677
GTATCGT47900.09.3982856
GTATCGA31300.09.2040614
TCGCATT83500.09.1856832
TATCGTT47100.09.1759267
CTCGCAT99150.09.1520611
TCGAAGG139300.08.9974147
CCCGTCG61750.08.8637551
CATCGAA114200.08.7977895
ACATCGA124850.08.6241524
CCGTTTA74000.08.6134144
CTCGGTT54450.08.5971911
TCGATCG62600.08.5944638
CTCGGAT88900.08.5465691
TACGCAC57950.08.477900550-51
Produced by FastQC (version 0.11.2)