FastQCFastQC Report
Sun 7 Aug 2016
GBM117.R2.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM117.R2.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74207368
Sequences flagged as poor quality0
Sequence length83
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT70700.011.2082731
CGTATCG40250.010.5598073
CTCGTAT66250.09.4057981
CTCCGAT107150.09.3788041
CTCGGTT109750.09.0253411
CTCGGAT152000.08.9811321
CCCGTCT238200.08.604111
CTCGCAT104100.08.4771681
CTCGAAT123200.08.4200991
GTCGGTT76600.08.3230011
CTCGGGT153550.08.2101981
CTGGAAT635800.08.2088681
CTTGGAT426150.08.071911
CCCGAAT125000.07.9530641
CGCCAAT82650.07.84449631
CTCGTTT127850.07.8321231
GTCCGAT45850.07.6987851
CCCGGAT159750.07.64353471
CTCGGCT294900.07.43837261
CGCGAAT18700.07.31943231
Produced by FastQC (version 0.11.2)