FastQCFastQC Report
Sun 7 Aug 2016
GBM12.R1.fastq.83bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM12.R1.fastq.83bp.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635321
Sequences flagged as poor quality0
Sequence length83
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGGT1350.036.980796
TCGTGCG1151.2732926E-1136.733437
CGCCCTT953.081368E-936.384841
TCAGACG854.7077265E-836.1412162
GATCGGA551.7342644E-434.9147451
CGGTAAA1450.034.4303939
TATCCGG1450.034.4303935
AGACGTG907.783092E-834.133374
TCCGGTA1500.033.282717
CCGGTAA1550.032.209088
TTATCCG1550.032.2064864
GCCAGTA3100.032.2064863
TAGCATA3050.031.4779748
CCAGTAG3200.031.2000314
TTACGAC1651.8189894E-1230.2570115
ACGAGCG1151.9894287E-830.0522083
GTTACGA1701.8189894E-1229.3647384
ACGTGGC1053.0070805E-729.259536
GTAGCAT3450.028.941497
CAGTAGC3500.028.528045
Produced by FastQC (version 0.11.2)