FastQCFastQC Report
Sun 7 Aug 2016
GBM120.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM120.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25792405
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT34050.010.5147931
GTATCGA25500.09.51124
GCGAACG11750.09.5015633
CGTATCG29100.08.996033
CCGTATC42550.08.1428772
CGCCTAT21150.08.0089811
ATCGAAG62850.07.96296366
CTCGGAT57150.07.40988351
GTCCGAT17400.07.3012911
CCCGGTT44850.07.29611061
CCGCAAT26200.07.1999541
CCCCCGT61000.07.1946551
CTCCGAT45200.07.069271
CGCGTTA27700.07.02038734-35
CCCGGGT108450.06.95763161
CCGGGAT78400.06.9236961
CGACCGT17800.06.9209371
CCCGCAT57900.06.9149611
GTTACGG34150.06.87663137
TATCGAA37950.06.7966975
Produced by FastQC (version 0.11.2)