FastQCFastQC Report
Sun 7 Aug 2016
GBM129.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGBM129.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35106781
Sequences flagged as poor quality0
Sequence length83
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT20100.09.7649891
CCCGTAT37150.08.909171
CTCCGAT58900.08.4289231
CTCGGAT73050.08.1659951
CGTATCG28300.08.1575743
TCGCATT55800.07.99917752
CGATTAG33500.07.81013354
GTCCGAT24800.07.75918251
GTCCGTT28450.07.5753621
CGGGGTT93450.07.45412871
CCCGGTT54850.07.4374981
CTCGCAT70850.07.33316661
CCGTATC42750.07.20071172
CCGGTAT67300.07.14813231
CTCCGGT66800.07.14402341
CGCGAAT8655.2948926E-67.11871341
CTCGGTT50600.06.99737171
CTCGTTT60900.06.9514121
CTCGAAT57300.06.9179951
CGCGTAA8958.786994E-66.8800971
Produced by FastQC (version 0.11.2)